| Autor: |
Zbrun, María V., Romero-Scharpen, Analía, Olivero, Carolina, Zimmermann, Jorge A., Rossler, Eugenia, Soto, Lorena P., Astesana, Diego M., Blajman, Jesica E., Berisvil, Ayelén, Frizzo, Laureano S., Signorini, Marcelo L. |
| Zdroj: |
Revista Argentina de Microbiologia; July-September 2017, Vol. 49 Issue: 3 p235-241, 7p |
| Abstrakt: |
The objective of this study was to investigate a clonal relationship among thermotolerant Campylobacterspp. isolates from different stages of the poultry meat supply chain in Argentina. A total of 128 thermotolerant Campylobacterspp. (89 C. jejuniand 39 C. coli) isolates from six poultry meat chains were examined. These isolates were from: a) hens from breeder flocks, b) chickens on the farm (at ages 1 wk and 5 wk), c) chicken carcasses in the slaughterhouse, and d) chicken carcasses in the retail market. Chickens sampled along each food chain were from the same batch. Campylobacterspp. isolates were analyzed using pulsed-field gel electrophoresis to compare different profiles according to the source. Clustering of C. jejuniisolates resulted in 17 profiles, with four predominant genotypes and many small profiles with just a few isolates or unique patterns, showing a very high degree of heterogeneity among the C. jejuniisolates. Some clusters included isolates from different stages within the same chain, which would indicate a spread of strains along the same poultry meat chain. Moreover, twenty-two strains of C. coliclustered in seven groups and the remaining 17 isolates exhibited unique profiles. Evidence for transmission of thermotolerant Campylobacterspp. through the food chain and cross contamination in the slaughterhouses were obtained. This collective evidence should be considered as the scientific basis to implement risk management measures to protect the public health. |
| Databáze: |
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