| Autor: |
Lynch, Shannon Colleen, Twizeyimana, Mathias, Mayorquin, Joey Sal, Wang, Danny Ho, Na, Francis, Kayim, Mukaddes, Kasson, Matthew T., Thu, Pham Quang, Bateman, Craig, Rugman-Jones, Paul, Hulcr, Jiri, Stouthamer, Richard, Eskalen, Akif |
| Zdroj: |
Mycologia; March 2016, Vol. 108 Issue: 2 p313-329, 17p |
| Abstrakt: |
Fusarium euwallaceaeis a well-characterized fungal symbiont of the exotic ambrosia beetle Euwallaceasp. (polyphagous shot hole borer [PSHB]), together inciting Fusarium dieback on many host plants in Israel and California. Recent discoveries of additional fungal symbionts within ambrosia beetle mycangia suggest these fungi occur as communities. Colony-forming units of Graphium euwallaceaesp. nov. and Paracremonium pembeumsp. nov., two novel fungal associates of PSHB from California, grew from 36 macerated female heads and 36 gallery walls collected from Platanus racemosa, Acer negundo, Persea americanaand Ricinus communis.Fungi were identified based on micromorphology and phylogenetic analyses of the combined internal transcribed spacer region (nuc rDNA ITS1-5.8S-ITS2 [ITS barcode]), elongation factor (EF 1-α), small subunit (18S rDNA) sequences for Graphiumspp., ITS, EF 1-α, calmodulin (cmdA), large subunit of the ATP citrate lyase (acl1), β-tubulin (tub2), RNA polymerase II second largest subunit (rpb2) and large subunit (28S rDNA) sequences for Paracremoniumspp. Other Graphiumspp. recovered from PSHB in Vietnam, Euwallacea fornicatusin Thailand, E. validusin Pennsylvania and Paracremoniumsp. recovered from PSHB in Vietnam were identified. F. euwallaceaewas recovered from mycangia at higher frequencies and abundances in all hosts except R. communis, in which those of F. euwallaceaeand P. pembeumwere equal. P. pembeumwas relatively more abundant within gallery walls of A. negundoand R. communis.In all hosts combined F. euwallaceaewas relatively more abundant within PSHB heads than gallery walls. All three fungi grew at different rates and colonized inoculated excised stems of P. americanaand A. negundo. P. pembeumproduced longer lesions than F. euwallaceaeand G. euwallaceaeon inoculated avocado shoots. Results indicate PSHB is associated with a dynamic assemblage of mycangial fungal associates that pose additional risk to native and nonnative hosts in California. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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