| Autor: |
Egesten, A, Alumets, J, von Mecklenburg, C, Palmegren, M, Olsson, I |
| Zdroj: |
Journal of Histochemistry and Cytochemistry; November 1986, Vol. 34 Issue: 11 p1399-1403, 5p |
| Abstrakt: |
An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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