| Abstrakt: |
We previously demonstrated that exposure of CCL39 lung fibroblasts to α-thrombin inhibits interleukin-6 (IL-6)-induced tyrosine phosphorylation of Stat3 (signal transducers and activators of transcription 3) via activation of mitogen-activated protein (MAP) kinase kinase 1 [Bhat et al. (1998) Arch. Biochem. Biophys. 350, 307–314]. In this study, using CCL39/MRC-5 cells, we investigated if additional signaling intermediates are involved in α-thrombin's inhibitory effects on IL-6-induced Stat3 signaling. We also determined if α-thrombin inhibits oncostatin M (OSM)-induced Stat3/Stat1, and interferon-γ (IFN-γ)-induced Stat1 tyrosine phosphorylation. We demonstrate that, although both IL-6 and OSM belong to the same cytokine family, α-thrombin inhibited only the IL-6-induced Stat3 tyrosine phosphorylation. The tyrosine phosphatase PTP1D coprecipitated with Stat3 from α-thrombin + IL-6, but not from α-thrombin + OSM-treated cells. Pretreatment of cells with a phosphatase inhibitor reversed the inhibitory actions of α-thrombin, suggesting a role for PTP1D in α-thrombin-mediated inhibition of IL-6-induced Stat3 signaling. Interestingly, α-thrombin failed to inhibit OSM- and IFN-γ-induced Stat1 tyrosine phosphorylation. Cytokine-specific inhibition of the Stat3 signaling involving MAP kinase kinase 1 and PTP1D by α-thrombin may play an important role in regulation of gene expression. |
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