| Autor: |
An, Zhenyi, Tassa, Amina, Thomas, Collin, Zhong, Rui, Xiao, Guanghua, Fotedar, Rati, Tu, Benjamin P, Klionsky, Daniel J, Levine, Beth |
| Zdroj: |
Autophagy; October 2014, Vol. 10 Issue: 10 p1702-1711, 10p |
| Abstrakt: |
In response to starvation, cells undergo increased levels of autophagy and cell cycle arrest but the role of autophagy in starvation-induced cell cycle arrest is not fully understood. Here we show that autophagy genes regulate cell cycle arrest in the budding yeast Saccharomyces cerevisiaeduring nitrogen starvation. While exponentially growing wild-type yeasts preferentially arrest in G1/G0in response to starvation, yeasts carrying null mutations in autophagy genes show a significantly higher percentage of cells in G2/M. In these autophagy-deficient yeast strains, starvation elicits physiological properties associated with quiescence, such as Snf1 activation, glycogen and trehalose accumulation as well as heat-shock resistance. However, while nutrient-starved wild-type yeasts finish the G2/M transition and arrest in G1/G0, autophagy-deficient yeasts arrest in telophase. Our results suggest that autophagy is crucial for mitotic exit during starvation and appropriate entry into a G1/G0quiescent state. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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