| Autor: |
Burney, Richard O., Lee, Alan I., Leong, Denise E., Jones, Joshua T., Hahn, Angela T., Meyer, Tobias, Yao, Mylene W.M. |
| Zdroj: |
Cell Cycle; September 2007, Vol. 6 Issue: 18 p2276-2283, 8p |
| Abstrakt: |
High content cell-based genetic and small molecule library screens are powerful strategies in drug discovery and investigations of disease mechanisms. We report that primary cells derived from a transgenic mouse model expressing a fluorescence mitosis biosensor provide unambiguous phenotype readouts without the need for transfection or immunocytochemistry. Phenotype profiles of cell cycle disruption and of apoptosis are easily detectable at a single time point selected from time-lapse live fluorescence microscopy. Most importantly, this transgenic mouse model may be crossed with cancer mouse models to derive biosensor-expressing primary cancer cells for use in high content screening strategies targeting discovery of tumor-specific chemotherapeutic compounds. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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