| Autor: |
Fukunaga, Rikiro, Matsuyama, Masako, Okamura, Hamki, Nagata, Kumiko, Nagata, Shigekazu, Sokawa, Yoshihiro |
| Zdroj: |
Nucleic Acids Research; June 1986, Vol. 14 Issue: 11 p4421-4421, 1p |
| Abstrakt: |
The relative levels of DNA methylation at CCGG sequences within and around the interferon-γ (IFN-γ) gene in normal human tissues and cell lines were examined by Southern blot analysis using isoschizomeric restriction enzymes, HpaII and MspI. On the test of normal tissues, the IFN-γ gene was under-methylated only in a small population of T lymphocyte, whereas the gene was fully methylated in T cell-depleted lymphocytes and uterus cells. In TCL-Fuj cell line which is a T cell line producing a high level of IFN-γ spontaneously, the IFN-γ gene was undermethylated. Moreover, the extent of DNA methylation was inversely correlated to the level of expression of the IFN-γ gene in several T cell lines including sublines derived from TCL-Fuj cells. However, partial or complete unmethylation at the CCGG sites of IFN-γ gene was observed in a promyelocytic leukemia cell line and two epithelial cell lines that fail to produce IFN-γ irrespective of induction. These results suggest that undermethy-lation of IFN-γ gene is necessary but not sufficient for its efficient expression. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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