Cultivation in vitroof Adult Human Skin and Oral Mucosa on Gelatin Film.∗

Autor: Smulow, Jerome B., Rustigian, Robert, Tye, Mauray
Zdroj: Experimental Biology and Medicine; April 1961, Vol. 106 Issue: 4 p757-762, 6p
Abstrakt: Zones of epithelioid cells from single fragments of adult human skin and oral mucosa develop on gelatin film. Although the initial appearance of these cells was somewhat slower on this substrate, their subsequent migration or growth appeared to be comparable to that in plasma clot. Proliferation of fibroblast-like cells was observed in some skin cultures on gelatin film when the medium consisted of a modified Eagle's basal solution supplemented with 15% calf serum but not when supplemented with 20% human or 10% human and 5% calf serum. Cultural characteristics of adult human skin and oral mucosa were similar except that fibroblast-like cells were more rarely observed and extensive sheaths of epithelioid cells occurred on glass as well as on gelatin film from oral mucosa.
Databáze: Supplemental Index