| Abstrakt: |
Ten strains of Mycoplasmawere isolated from brain tissues of a scrapie infected sheep and mouse, joint exudate of an arthritic goat, and cell cultures established from human, chimpanzee, and dog tissues. These strains were related to each other, but were unrelated to known species of Mycoplasma.The present report characterizes the new strains, proposes that they be classified as Mycoplasma arigininiand discusses their ecological significance.Materials and Methods. Media for isolation.Hayflick (1), BYE (2), and BBL (3) broth and agar media were used under aerobic and anaerobic (5% CO2in nitrogen) conditions (2). See Table I for media used for isolation of individual strains.Media for metabolic reactions. Arginine utilization reaction:Hayflick and BYE broth media were supplemented with 10 mMarginine, 10 mMglutamine, vitamins (4), 0.002% phenol red, and adjusted to pH 7.1.Glucose fermentation reaction:Hayflick broth medium was supplemented with 0.5% glucose, 10 mMglutamine, vitamins, 0.004% cresol red, and adjusted to pH 7.5.Urea metabolic reaction:Hayflick broth medium was supplemented with 0.5% urea, 0.002% phenol red, and adjusted to pH 6.0. Utilization of arginine and urea was indicated by an alkaline shift in pH while fermentation was indicated by an acid shift in pH.Isolation of strains.Source and origin of specimens from which 10 strains were isolated are given in Table I. Two strains were isolated from brain tissues of two animals infected with scrapie, 1 from a sheep (C506) with naturally acquired scrapie and the other from a mouse (G230) with scrapie experimentally induced by the intracerebral injection of a brain suspension from another sheep with naturally acquired scrapie. The latter suspension was not available for direct culturing for Mycoplasma.These 2 Mycoplasmastrains were isolated by direct culture in both BYE and Hayflick broth and agar media aerobically and anaerobically. |
