| Autor: |
Boll, Meinrad, Weber, Lutz W. D., Becker, Eberhard, Stampfl, Andreas |
| Zdroj: |
Zeitschrift für Naturforschung C; February 2001, Vol. 56 Issue: 1-2 p111-121, 11p |
| Abstrakt: |
The CCl4-induced development of liver damage was studied in monolayer cultures of primary rat hepatocytes: (1) CCl4caused accumulation of triglycerides in hepatocytes following cytochrome P450 induction with ß-naphthoflavone or metyrapone. Ethanol or a high dose of insulin plus triiodothyronine had the same effect. (2) CCl4increased the synthesis of fatty acids and triglycer ides and the rate of lipid esterification. Cholesterol and phospholipid synthesis from acetate was also increased. (3) CCl4reduced ß-oxidation of fatty acids as assessed by CO2-release and ketone body formation. Hydrolysis of triglycerides was also reduced. (4) The content of unsaturated fatty acids in microsomal lipids was decreased by almost 50% after incubation with CCl4, while saturated fatty acids increased slightly. (5) CCl4exerted a pronounced inhibitory effect on the exocytosis of macromolecules (albumin), but did not affect secretion of bile acids from hepatocytes. |
| Databáze: |
Supplemental Index |
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