| Autor: |
Délot, Emmanuèle, Kataoka, Hiroko, Goutel, Carole, Yan, Yi-Lin, Postlethwait, John, Wittbrodt, Jochen, Rosa, Frédéric M |
| Zdroj: |
Mechanisms of Development; July 1999, Vol. 85 Issue: 1-2 p15-25, 11p |
| Abstrakt: |
We have previously cloned several members of the TGF-βsuperfamily of growth factors in zebrafish, one of which, Radar, belongs to the Dpp-Vg1-related (DVR) subgroup, with highest homology to GDF6. The pattern of expression of Radarsuggested a possible involvement in several induction steps during embryogenesis including in the dorsal neural tube, red blood cells, the dorsal fin and the retina. We have analyzed the pattern of expression of Radarin comparison with that of a marker of dorsal neural tube structures, msxCand show that Radarand msxCare expressed in similar and/or adjacent tissues throughout embryogenesis. In order to demonstrate a functional relationship between these two proteins, we have generated a full-length cDNA for Radarand shown that Radaroverexpression by DNA injection maintains expression of msxCin tissues where it is normally expressed then turned off, in particular in the dorsal neurectoderm. Study of the phenotype of a mutant carrying a deletion of Radarshows a loss of identity and death of the cells of the dorsal neural tube. Taken together these results suggest that Radarcould be involved in maintaining the identity of cells of the dorsal-most neural tube and of at least a subset of neural crest cells. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect