| Autor: |
Peng, Hongqi, Xu, Fengji, Pershad, Rashmi, Hunt, Kelly K., Frazier, Marsha L., Berchuck, Andrew, Gray, Joe W., Hogg, David, Bast, Robert C., Yu, Yinhua |
| Zdroj: |
International Journal of Cancer; June 2000, Vol. 86 Issue: 5 p690-694, 5p |
| Abstrakt: |
In our previous work, we had characterized ARHIas an imprinted putative tumor‐suppressor gene in ovarian and breast cancers. ARHIis expressed in primary breast and ovarian cell lines but largely absent from the corresponding malignant tumors. Moreover, the non‐imprinted functional allele is typically deleted in malignant cells. Since ARHIhad been mapped to 1p31, a common deletion site in breast and ovarian cancer and male germ‐cell tumors, in this study, we set out to define precisely the physical location of ARHIat 1p31 and to determine if this location lies within the smallest common region of deletion in breast and ovarian cancers. To this end, we first carried out radiation hybrid mapping of ARHIand surrounding markers, followed by a high‐resolution study of loss of heterozygosity at 1p31 in 49 ovarian and breast cancers. Combining a radiation hybrid map and a physical map of the region encompassing ARHI,3 discrete regions of minimal deletion were found at 1p31 in breast and ovarian cancers. ARHIis the most common deletion region at 1p31. Two other less common regions of deletion were found centromeric to this gene. One of them centered on D1S207 and the other one included and was proximal to D1S488. We also confirmed the preferential loss of non‐imprinted functional allele in 7 of 9 tumor specimens. These data support the possibility that ARHIis a tumor‐suppressor gene and suggest that additional tumor‐suppressor genes may lie proximal to ARHIat 1p31. The data obtained from our study should aid in the identification and characterization of genes in this novel imprinted region. Int. J. Cancer86:690–694, 2000. © 2000 Wiley‐Liss, Inc. |
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