| Autor: |
SHIBA, Yoichiro, ONO, Chiho, ICHIKAWA, Kimihisa, SERIZAWA, Nobufusa, YOSHIKAWA, Hiroji |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; January 1999, Vol. 63 Issue: 8 p1414-1419, 6p |
| Abstrakt: |
The production level of CPY in Saccharomyces cerevisiaeKS58-2D/pCY303 was drastically decreased when thiamine was not added to the culture medium. We isolated and characterized the mutants that could produce CPY even though thiamine was absent from the medium. Using complementation screening in the mutants obtained, we isolated a gene that was involved in the thiamine-inducible expression, TIE1, which corresponded to the YDR325wORF on chromosome IV. The predicted protein sequence of TIE1did not have significant homology to proteins from public databases. The disruption of the TIE1gene caused two phenotypes, increase of expression level in thiamine-free medium and ethanol sensitivity. This increase in thiamine-free medium was also observed in the expression under the control of ENO1or ADH1promoter in addition to the GAL10promoter, suggesting that the TIE1 protein is associated with a similar kind of transcriptional mechanism regulated by thiamine. |
| Databáze: |
Supplemental Index |
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