| Autor: |
SHIBA, Yoichiro, ONO, Chiho, FUKUI, Fumio, WATANABE, Ichiro, SERIZAWA, Nobufusa, GOMI, Katsuya, YOSHIKAWA, Hiroji |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; January 2001, Vol. 65 Issue: 1 p94-101, 8p |
| Abstrakt: |
Phospholipase A1(PLA1) is a hydrolytic enzyme that catalyzes the removal of the acyl group from position 1 of lecithin to form lysolecithin. The PLA1gene, which had been cloned from Aspergillus oryzae, was expressed in Saccharomyces cerevisiae and A. oryzae. Through the modification of the medium composition and the feeding conditions of substrate, the production level of PLA1by S. cerevisiae was increased to a level fivefold higher than that indicated in a previous report. In the case of A. oryzae, introduction of multicopies of PLA1expression units, and the morphological change from the pellet form to the filamentous form were effective for the enhancement of PLA1production. We succeeded in producing 3,500 U/ml of PLA1using an industrial-scale fermentor. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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