| Autor: |
Mackey, Joseph R., Melendez, Elsa L. Vazquez, Farrell, John J., Lowery, Kristin S., Rounds, Megan A., Sampath, Rangarajan, Bonomo, Robert A. |
| Zdroj: |
Journal of Clinical Microbiology; June 2014, Vol. 52 Issue: 6 p2259-2261, 3p |
| Abstrakt: |
ABSTRACTWe describe the evaluation of culture-negative synovial fluid from a 3-year-old boy by PCR and electrospray ionization followed by mass spectrometry (PCR/ESI-MS). Our patient developed a diffuse rash and fever with systemic signs and symptoms of sepsis, but four sets of blood cultures obtained prior to initiation of antibiotics were negative. After 1 week of illness, he developed right-knee swelling. Analysis of synovial fluid was consistent with infection, but cultures of specimens obtained following initiation of antimicrobial treatment were negative for growth. PCR/ESI-MS detected Streptobacillus moniliformisin the synovial fluid sample. Our patient completed an appropriate course of antibiotic treatment and remained completely asymptomatic in follow-up evaluation. This unique case suggests that PCR/ESI-MS may be a useful diagnostic tool for direct detection of unusual or unexpected pathogens directly from clinical specimens, particularly when samples have been obtained from patients following initiation of antibiotic therapy. |
| Databáze: |
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