| Autor: |
Brereton, Richard G, Devonshire, Martin |
| Zdroj: |
Analyst; 2004, Vol. 129 Issue: 3 p249-253, 5p |
| Abstrakt: |
This paper describes a method for genetic screening using single nucleotide polymorphism. Fluorescence spectra with an excitation frequency of 488 nm are recorded over a range of 550 to 660 nm of fragments of human DNA together with two fluorescent probe dyes attached to specific primers, one for each type of allele and a background dye, prepared using the Taqman reaction. The fluorescence spectra are monitored and principal components analysis used to separate spectra into three groups, which are visually identified as allele 1 wild type, allele 2 mutant and mixed allele by comparison to reference samples. Malahanobis distance using 4 principal components are used to correctly classify samples into groups. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
