| Autor: |
Curley, Peter, Sinderen, Douwe |
| Zdroj: |
FEMS Microbiology Letters; February 2000, Vol. 183 Issue: 1 p177-182, 6p |
| Abstrakt: |
Analysis of the sequence of a randomly cloned chromosomal DNA fragment (3.2 kb) from Lactococcus lactisrevealed the presence of part of an open reading frame, designated amd1, which specifies a protein displaying significant similarity to aminoacylases from various bacteria. The presence of an immobilised copy of an IS982element immediately upstream of the coding region of amd1has probably resulted in the displacement of amd1's native promoter. This genetic organisation was shown to be retained in seven other dairy strains, one of which was only slightly different. The amd1gene was overexpressed in L. lactisNZ9800 under the control of the inducible nisApromoter and the deacetylating capacity of its gene product was measured on a number of substrates. |
| Databáze: |
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