| Abstrakt: |
Recently a new resorbable PLACL monofilament suture made of Llactide and caprolactone was developed. The sensitization test was carried out using a guinea pig maximization test according to the Japanese Guidelines. As a result of this test, it was revealed that the methanol extract of PLACL suture contains a skin sensitizer. It was suggested that the D&C Violet No. 2 contained in PLACL sutures is decomposed during the meltspinning process and that the decomposed dye induces skin sensitization. To characterize the sensitizer the decomposed dye it was separated and collected by HPLC. To determine the molecular formula of the sensitizer, MS, IR, and NMR spectra were obtained. The molecular weight of the sensitizer was estimated as 240 by measuring the MS. Considering the chemical structure of the dye and the molecular weights, it was determined that the sensitizer was 1,4dihydroxyanthraquinone quinizarin. The D&C Violet No. 2 contained in PLACL sutures was decomposed by the meltspinning procedure to form quinizarin, one type of anthraquinone dye, and the sensitizer was identified to be, indeed, quinizarin. To evaluate the sensitizing potential of quinizarin, we tested skin sensitization using the maximization test method. Quinizarin induced skin sensitization in guinea pigs, and the minimum sensitizing concentration was approximately 100 ppm. The concentration of quinizarin contained in the PLACL suture was 1–5 ppm, a value that is lower than the minimum sensitizing concentration of quinizarin. This suggests that PLACL suture has a low risk with regard to contact dermatitis. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 531–539, 2001 |
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