| Autor: |
Müller, Tobias, Benjdia, Mariam, Avolio, Meghan, Voigt, Boris, Menzel, Diedrik, Pardo, Alejandro, Frommer, Wolf, Wipf, Daniel |
| Zdroj: |
Mycorrhiza; September 2006, Vol. 16 Issue: 6 p437-442, 6p |
| Abstrakt: |
Hebeloma cylindrosporumis a model fungus for mycorrhizal studies because of its fast growth rate, simple nutritional requirements, and completion of its life cycle in vitro, and because it is amenable to transformation. To advance cell biological research during establishment of symbiosis, a tool that would enable the direct visualisation of fusion proteins in the different symbiotic tissues [namely, the expression of reporter genes such as Green Fluorescent Protein (GFP)] was still a missing tool. In the present study, H. cylindrosporumwas transformed using Agrobacteriumcarrying the binary plasmid pBGgHg containing the Escherichia colihygromycin B phosphotransferase (hph) and the EGFP genes, both under the control of the Agaricus bisporusglyceraldehyde-3-phosphate dehydrogenase promoter. EGFP expression was successfully detected in transformants. The fluorescence was uniformly distributed in the hyphae, while no significant background signal was detected in control hyphae. The suitability of EGFP for reporter gene studies in Hebeloma cylindrosporumwas demonstrated opening up new perspectives in the Hebelomagenetics. |
| Databáze: |
Supplemental Index |
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