| Autor: |
MZali, Fatima-Hannachi, Gascoyne-Binzi, Deborah M., Heritage, John, Hawkey, Peter M. |
| Zdroj: |
Journal of Antimicrobial Chemotherapy (JAC); April 1996, Vol. 37 Issue: 4 p797-802, 6p |
| Abstrakt: |
Single strand conformational polymorphism (SSCP) is a recently developed technique used to detect single base mutations in short PCR-generated amplimers. The method has been adapted and applied to differentiation of β-lactamase genes. Each of the five standard SHV strains used produced a unique SSCP pattern, allowing the possibility of rapid identification of the SHV genes of other isolates. A clinical isolate that phenotypically produced SHV-5 yielded a pattern of major bands indistinguishable from that of the SHV-5 standard strain, illustrating the applicability of this technique. We therefore report a reliable and reproducible technique that can be applied to the characterisation of the SHY β-lactamases. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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