| Autor: |
Sulzer, D, St Remy, C, Rayport, S |
| Zdroj: |
Molecular Pharmacology; February 1996, Vol. 49 Issue: 2 p338-342, 5p |
| Abstrakt: |
Although amphetamine releases catecholamines from isolated secretory vesicles, a number of in vivo experiments have indicated that the vesicular amine transport blocker reserpine does not block amphetamine-induced release. To address this paradox, we examined the effect of reserpine on amphetamine-induced dopamine release from postnatal ventral midbrain neurons in culture. These cultures provide a preparation in which intracellular, extracellular, and releasable dopamine pools can be measured simultaneously. We found that 1 microM reserpine for 90 min reduced stimulation-dependent dopamine release by > 95%. In parallel, reserpine reduced amphetamine-induced dopamine release by > 95% compared with cells not exposed to reserpine or by 75% compared with reserpine-treated cultures. This shows that amphetamine acts principally by redistributing dopamine from synaptic vesicles to the cytosol. |
| Databáze: |
Supplemental Index |
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