Transfer of Ti plasmids between Agrobacterium strains by mobilisation with the conjugative plasmid RP4

Autor: Larebeke, N., Genetello, Ch., Hernalsteens, J. P., Picker, A., Zaenen, I., Messens, E., Montagu, M., Schell, J.
Zdroj: Molecular and General Genetics MGG; January 1977, Vol. 152 Issue: 2 p119-124, 6p
Abstrakt: The P type conjugative plasmid RP4 has been shown to be able to promote the transfer of the Agrobacterium Ti-plasmid. The results provide additional evidence that agrocin 84 sensitivity, exclusion of phage AP1, ability to catabolize the guanidine derivatives octopine and nopaline and tumor inducing ability, are Ti-plasmid determined properties. Furthermore, the results strongly support the notion that at least part of the Ti-plasmid is transferred from the bacterium to the target plant cells, since it was demonstrated that Ti-plasmid linked genes specify the synthesis of octopine or nopaline by crown-gall tumor cells.
Databáze: Supplemental Index
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