The effect of hypoxia on the levels of circulating catecholamines in the dogfishScyliorhinus canicula

Autor: Butler, P. J., Taylor, E. W., Capra, M. F., Davison, W.
Zdroj: Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology; December 1978, Vol. 127 Issue: 4 p325-330, 6p
Abstrakt: 1.A radio-enzymatic technique was used to assay the catecholamines, dopamine (DA), noradrenaline (NA) and adrenaline (A) in relatively small samples of dogfish blood, obtained from an indwelling cannula. The removal of a 2 ml sample of blood has no appreciable effect on the levels of circulating catecholamines in the dogfish. Thus, the three amines could be measured before and after a particular experimental treatment by removing 2 ml of blood before and 7–8 ml of blood after the treatment.2.In resting, normoxic dogfish at 15°C, the mean (±S.E.) levels of the three amines in the plasma were: DA, 6.0±1.2 pmol ml-1; A, 25.6±6.5 pmol ml-1; NA, 32.1±19.3 pmol ml-1. Following exposure to an inspired oxygen tension of approximately 35 mmHg for 1.5 h there was an increase in all three catecholamines to: DA, 11.3±1.4 pmol ml-1; A, 284.4±86.2 pmol ml-1; NA, 446.5±117.6 pmol ml-1. These increases are discussed in terms of the known effects of the substances on the isolated heart and branchial arteries of elasmobranchs.
Databáze: Supplemental Index