| Autor: |
Zhongyi, Li, Tanner, G. J., Larkin, P. J. |
| Zdroj: |
Plant Cell, Tissue and Organ Culture; April 1990, Vol. 21 Issue: 1 p67-73, 7p |
| Abstrakt: |
Seedling and suspension culture protoplasts of subterranean clover (Trifolium subterraneum L.) were successfully cultured in semi-solid drops of calcium alginate and ultrafiltered liquid medium. Protoplast-derived subterranean-clover colonies developed as the osmolality was lowered over three steps. Callus was established from these colonies. Calli derived from protoplasts have failed to regenerate on a range of media. The frequency of dividing subterranean-clover protoplasts was increased in the presence of lucerne (Medicago sativa L.) nurse cells. Low-voltage treatments (200 mV) for the first 16–132 hours of culture also resulted in a 100% increase in the frequency of dividing protoplasts. |
| Databáze: |
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| Externí odkaz: |
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