| Autor: |
Sapp, R. J., Christianson, J., Stark, W. S. |
| Zdroj: |
Journal of Neurocytology; July 1991, Vol. 20 Issue: 7 p597-608, 12p |
| Abstrakt: |
Summary Electron microscopy was used to investigate membrane turnover in the photoreceptors ofDrosphila. Coated pits and vesicles, multivesicular bodies, primary lysosomes, multilamellate bodies, residual bodies and Golgi complexes are present throughout a light/dark cycle. Serial sections reveal that the membrane bounding of multivesicular bodies is only seen at an optimal plane of section. The temperature-sensitiveshibire (shits) mutant has a defect in conversion of coated pits into vesicles which may also affect visual receptors. We used monoclonal antibodies to Rhl in Rl-6 receptors in the compound eye (also to Rh2 inocellar receptors in the simple eyes) ro relate turnover processes at the visual pigment compared with membrane levels. Compound eye rhabdomeres but not rhabdomere caps stained selectively. Immunogold labelling was equivocal in multivesicular bodies. Further, early in the process of carotenoid replacement therapy, labelling is high in the rough endoplasmic reticulum, demonstratingde novo opsin synthesis. |
| Databáze: |
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