Autor: |
Nagarajan, Venugopalan, Sakurai, Nobuyuki, Kubota, Miho, Nonaka, Takamasa, Nagumo, Hiroaki, Takeda, Hisashi, Nishizaki, Tomoko, Masai, Eiji, Fukuda, Masao, Mitsui, the late Yukio |
Zdroj: |
Protein and Peptide Letters; August 2003, Vol. 10 Issue: 4 p412-417, 6p |
Abstrakt: |
The terminal oxygenase component of the biphenyl dioxygenase (BphA1A2 complex) was over-expressed with a novel over expression system in recombinant Rhodococcus strain and purified. The purified enzyme has been crystallized by the hanging drop vapor diffusion method and subjected to X-ray diffraction analysis. The crystals belong to the tetragonal system in the space group P4 1 2 1 2 or P4 3 2 1 2 and diffract to better than 2.2Å resolution. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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