| Abstrakt: |
A methyl green-thionine stain was developed which gives a fairly selective staining of nucleic acids and a sharp differential coloring of chromatin, nucleoli, and basophil cytoplasm. Ribonucleic acid stains red, metachromatically; and DNA, blue-green. Proteins and mucins do not stain. Only the polysaccharides of mast cell granules and cartilage stain, also metachromatically. These substances can be distinguished from RNA by enzymic digestion tests, or more easily, by using the stain at pH 1.2; at this low pH nucleic acids do not stain. |
