| Autor: |
Ye, Zi-Qing, Qiu, Ping, Burkholder, Joseph K., Turner, Joel, Culp, Jerilyn, Roberts, Tim, Shahidi, Nasrollah T., Yang, Ning-Sun |
| Zdroj: |
Human Gene Therapy; October 1998, Vol. 9 Issue: 15 p2197-2205, 9p |
| Abstrakt: |
ABSTRACTInduction or short-term transgenic expression of specific cytokines, growth factors, or other candidate therapeutic genes in hematopoietic progenitor or stem cells is potentially applicable to gene therapy for cancer. In this study, we explored the application of a gene gun technique, as an alternative to viral vectors, for ex vivogene transfer into and transient gene expression in highly enriched CD34cells derived from human umbilical cord blood. Twenty-four hours posttransfection, 32.6 to 1500 pg/1 106CD34cells of transient gene expression was routinely obtained for specific cytokine and reporter genes. Transgene expression at the single-cell level was revealed by X-Gal staining of lacZcDNA-transfected CD34cells. Expression of four candidate therapeutic genes, namely human granulocyte-macrophage colony-stimulating factor, tumor necrosis factor , interleukin 2, and interferon , was detectable for 4 to 7 days in CD34cells. A human elongation factor 1promoter/intron 1 transcription unit was identified as a strong cellular promoter for CD34cells, exhibiting strength similar to that of the commonly employed cytomegalovirus immediate-early promoter. These results suggest that the nonviral, gene gun technique offers an efficient alternative approach for transient transgenic studies of hematopoietic cells and may provide new possibilities for certain cancer gene therapy strategies using CD34cells. |
| Databáze: |
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