| Autor: |
Andreoletti, Marion, Pags, Jean-Christophe, Mahieu, Dominique, Loux, Nathalie, Farge, Denis, Sacquin, Paul, Simon, Lionel, Hamza, Jamil, Bargy, Frdric, Briand, Pascale, Leperq, Jacques, Weber, Anne |
| Zdroj: |
Human Gene Therapy; February 1997, Vol. 8 Issue: 3 p267-274, 8p |
| Abstrakt: |
ABSTRACTFetal hepatocytes are an attractive target for in uterocellular transplantation. Their use could provide a very efficient way for implanting normal or transduced cells into the livers of affected fetuses. Marking cells with recombinant retroviruses is a powerful tool for evaluating the chimerism of grafted animals. The technique relies on the ex vivotransduction efficiency of the engrafted cells. We have isolated fetal primary hepatocytes from nonhuman primates. The cells were cultured and transduced with a retroviral vector carrying the Escherichia coli -galactosidase gene. Optimal gene transfer efficiency was obtained 4860 hr after plating and was as high as 90%. Cryopreservation had little effect on cell viability and infectivity: The viability of thawed hepatocytes remained high (7585%) and the infection efficiency was identical to that of freshly isolated cells. Efficient ex vivoretroviral gene transfer into fetal hepatocytes provides an appropriate system for testing allogenic grafting and for modifying immunogenicity of engrafted cells. These results open up new perspectives for cell transplantation through cell banking. |
| Databáze: |
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