| Autor: |
Lew, Denise, Parker, Suezanne E., Latimer, Terrie, Abai, Anna M., Kuwahara-Rundell, Ann, Doh, Soeun Grace, Yang, Zhi-Yong, Laface, Drake, Gromkowski, Stanislaw H., Nabel, Gary J., Manthorpe, Marston, Norman, Jon |
| Zdroj: |
Human Gene Therapy; May 1995, Vol. 6 Issue: 5 p553-564, 12p |
| Abstrakt: |
ABSTRACTThe fate of plasmid DNA complexed with cationic lipids delivered intravenously in mice was evaluated at selected timepoints up to 6 months postinjection. Blood half-life and tissue distribution of plasmid DNA and potential expression in tissues were examined. Southern blot analyses of blood indicated that intact plasmid DNA was rapidly degraded, with a half-life of less than 5 min for intact plasmid, and was no longer detectable at 1 hr postinjection. Southern analyses of tissue demonstrated that intact DNA was differentially retained in the lung, spleen, liver, heart, kidney, marrow, and muscle up to 24 hr postinjection. After 7 days, no intact plasmid DNA was detectable by Southern blot analysis; however, the plasmid was detectable by the polymerase chain reaction (PCR) in all tissues examined at 7 and 28 days postinjection. At 6 months postinjection, femtogram levels of plasmid were detected only in muscle. Immunohistochemical analyses did not detect encoded protein in the tissues harboring residual plasmid at 1 or 7 days postinjection. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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