Interspecific somatic hybridization betweenSolanum tuberosumL. andS. bulbocastanumdun. as a means of transferring nematode resistance

Autor: Austin, S., Pohlman, J. D., Brown, C. R., Mojtahedi, H., Santo, G. S., Douches, D. S., Helgeson, J. P.
Zdroj: American Journal of Potato Research; June 1993, Vol. 70 Issue: 6 p485-495, 11p
Abstrakt: Interspecific somatic hybrids were produced between tetraploidSolanum tuberosumand a nematode-resistant accession of the diploid speciesSolanum bulbocastanumby protoplast fusion. Hybrid cells were selected using dual fluorescent labeling of protoplast preparations prior to fusion. Hybridity of regenerated plants was confirmed with a combination of morphological assessment, chromosome counting and isozyme analysis. Somatic hybrids had the same level of resistance to infection by race 1 of the nematodeMeloidogyne chitwoodias theS. bulbocastanumparent used in the fusion. Some of the somatic hybrids were fertile as females when crossed with tetraploidS. tuberosumbreeding lines. Thus, these hybrids can be used in a potato improvement program to incorporate a valuable pest resistance
Databáze: Supplemental Index