| Autor: |
Gort, Steven M., van der Hoff, G. Rene, Baumann, Robert A., van Zoonen, Piet, Martin‐Moreno, Jose M., van't Veer, Piet |
| Zdroj: |
Journal of High Resolution Chromatography (now called Journal of Separation Science); March 1997, Vol. 20 Issue: 3 p138-142, 5p |
| Abstrakt: |
Long‐term exposure to fat‐soluble xenobiotics is assessed by the concentration of DDE [1,1‐dichloro‐2,2‐bis(p‐chlorophenyl)ethyl‐ene], the persistent metabolite of DDT, in subcutaneous fat, aspirated from the buttocks of breast cancer patients and age‐matched controls, from five European centers collaborating in a case‐control study on breast cancer. In such studies using sample material of living subjects only small amounts of samples can be made available for analysis. In this particular study the only sample material available for the analysis of DDE were aliquots of the aspirates that were originally analyzed for fatty acids. Due to the small sample quantities available, e.g. aliquots of 200–800 p. 1, on‐line LC‐GC is most convenient because a major part of the sample can be used in the analytical procedure. In the LC‐GC procedure 50 μl of sample was injected on the LC column resulting in a 180 μl fraction containing the analytes of interest. The LC fraction was transferred to a capillary GC with electron capture detector by means of partially concurrent solvent evaporation. This way, sample handling is minimized thus reducing losses and preventing contamination. The feasibility of the on‐line LC‐GC system for the analysis of DDE is demonstrated with the analysis of 634 adipose tissue extracts in different series. The validity of this approach, using samples already analyzed for another parameter, with LC‐GC is clearly demonstrated by the fact that in over 97% of the samples DDE can be quantified. Thus rendering a meaningful data‐set for further epidemiological evaluation. The DDE levels found ranged from 0.99–3.13 μg/g adipose tissue. |
| Databáze: |
Supplemental Index |
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