| Autor: |
Weber, Peter C., Challberg, Mark D., Nelson, Nancy J., Levine, Myron, Glorioso, Joseph C. |
| Zdroj: |
Cell; July 1988, Vol. 54 Issue: 3 p369-381, 13p |
| Abstrakt: |
The bacterial transposable element Tn5 was observed to undergo high-frequency sequence inversion when integrated into the herpes simplex virus type 1 (HSV-1) genome. Deletion analysis of the IS50 elements through which this recombination event occurred demonstrated the absence of cis-acting signals involved in the inversion process. Several observations suggested an intimate association of the recombination mechanism with HSV-1 DNA replication, including the ability of the seven viral genes that are essential for HSV-1 DNA synthesis to mediate Tn5 inversion in the absence of any other viral functions. Comparable results were obtained by using duplicate copies of the L-S junction of the HSV-1 genome. Thus inversion of the L and S components of the HSV-1 genome during productive infection does not appear to be a site-specific process, but rather is the result of generalized recombination mediated by the complex of gene products that replicate the viral DNA. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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