| Autor: |
Kojima, Naoshi, Sugino, Maiko, Mikami, Akiko, Nonaka, Ken, Fujinawa, Yumi, Ueda, Yoji, Sato, Kousuke, Ohtsuka, Eiko, Matsubara, Kenichi, Komatsu, Yasuo |
| Zdroj: |
Nucleic Acids Symposium Series; September 2005, Vol. 49 Issue: 1 p181-181, 1p |
| Abstrakt: |
We report here a new amino-modifier reagent, which enable high-throughput purification of amino-modified oligonucleotides. Either monomethoxytrityl (MMT) or trifluoroacetyl (TFA) group has been used as the protecting group for the primary amine when amino-terminal oligonucleotides are prepared. Generally, the removal of MMT requires the stringent acidic treatment after the oligonucleotide synthesis. We chemically synthesized a novel amino-modifier with the MMT protection. It was found that the new amino-modifier released MMT group under mild acidic condition, and then rapid purification of diverse amino-modified oligonucleotides could be achieved with cartridge column of reverse phase. Furthermore, we tried to construct the new detection system to study gene expression using the amino-modified oligonucleotides. The new amino-modifier will be useful for molecular biology by facilitating the construction of oligonucleotide library and improving the chemical reactivity. |
| Databáze: |
Supplemental Index |
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