| Autor: |
Atassi, M. Zouhair, Berliner, Lawrence J., Chang, Rowen Jui-Yoa, Jörnvall, Hans, Kenyon, Geroge L., Wittman-Liebold, Brigitie, Uversky, Vladimir N., Fink, Anthony L., Kuroiwa, Mie, Aoki, Kimiko, Izumiyama, Naotaka |
| Zdroj: |
Protein Misfolding, Aggregation & Conformational Diseases (978-0-387-25918-5); 2006, p277-283, 7p |
| Abstrakt: |
The localization of amyloid fibril components and the cells related to the formation and resorption of the fibrils are still controversial. In this study we undertook a time-kinetic study to analyze the process of amyloid fibril deposition in the spleen of AA amyloidosis animal model immunohistochemically and ultrastructurally. Murine amyloid A (AA) amyloidosis was induced by the emulsion injection composed of Freund's complete adjuvant and Mycobacterium butyricum. Serum amyloid A (SAA) level was the highest at 3 days after the induction and gradually decreased. The amyloid deposition was first detected in extracellular spaces in the marginal zone of the spleen at 7 days after induction. The F4/80 positive red pulp macrophages increased in number after the induction and accumulated near the amyloid deposition areas. Amyloid P component (APC) and chondroitin sulfate proteoglycan (CSPG), which are composed of amyloid fibril, were detected in the cytoplasm of F4/80 positive red pulp macrophages and ER-TR9-positive marginal zone macrophages, respectively, then localized in the amyloid deposition areas. APC was also localized in CSPG-positive and F4/80-negative cells, which might be fibroblasts at 3 days. Ultrastructural examination indicated that macrophages in the marginal zone contained lysosome-derived fibrillar structures of amyloid, and that fibroblasts extended amyloid fibrils into the extracellular area in the marginal zone. These results suggested the close association of APC-positive/ER-TR9-positive macrophages and APC-positive/CSPG-positive fibroblasts with the formation of amyloid fibrils and F4/80-positive macrophages with the resorption of the fibrils. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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