| Autor: |
Smith, Rodney, Farges, B., Tessier, B., Chevalot, I., Harscoat, C., Chenu, S., Marc, I., Goergen, J.L., Marc, A. |
| Zdroj: |
Cell Technology for Cell Products; 2007, p663-665, 3p |
| Abstrakt: |
To limit contamination risks by animal-derived components, we designed a new serum-free medium supplemented with rapeseed peptidic fractions. Compared to a reference serum-free medium this new medium allows a 3-fold increase in cell density, a 2-times extended longevity, a 5 times higher final product titer, Modifications of glucose metabolism were also observed. Addition of free amino-acids did not lead to the same positive effect, suggesting that peptides act not only as carbon, nitrogen and energy source but also as growth or survival factors. This rapeseed fraction also allows a very simple formulation of a protein-free medium. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
