| Autor: |
Walker, John M., Rayne, Richard C., Holman, G. Mark, Hayes, Timothy K. |
| Zdroj: |
Neurotransmitter Methods; 1997, p205-218, 14p |
| Abstrakt: |
Coupled with solid-phase extraction methodology, reverse-phase high performance liquid chromatography (HPLC) has taken peptide isolation and purification from the very difficult to the almost routine. When combined with an efficient detection system (bioassay, ELISA, RIA, MALDI-MS), appropriate HPLC purification systems can provide peptides of sufficient purity for sequence analysis and structural characterization in a relatively short time. Despite these technical advances, the unique characteristics of the target peptide for any isolation campaign still will provide the investigator with a special challenge or two in adapting the individual methods discussed in this chapter. For those investigators interested in additional information and tips on peptide and protein separation and purification by HPLC, the authors would suggest obtaining the booklet listed in the references (1). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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