| Autor: |
Agematu, Hitosi, Matsumoto, Naoki, Fujii, Yoshikazu, Kabumoto, Hiroki, Doi, Satoru, Machida, Kazuhiro, Ishikawa, Jun, Arisawa, Akira |
| Předmět: |
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| Zdroj: |
Bioscience, Biotechnology & Biochemistry; Jan2006, Vol. 70 Issue 1, p307-311, 4p, 1 Diagram, 1 Chart, 1 Graph |
| Abstrakt: |
The article presents a study of a bacterial P450-expression system with Escherichia coli. E. coli has no P450 genes its its genome, the organism is a suitable host for characterization of P450. It was transformed with expression vector pET11a carrying a P450 gene and camAB. The expression of P450 in E. coli was also confirmed by their spectral characterization. The UV-visible absorption spectrum of the supernatant of ruptured cell contained the characteristic 390-450 nanometer P450 peak. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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