| Autor: |
César BRAVO-REYNA, Carlos, ZENTELLA, Alexandro, Luis VENTURA-GALLEGOS, José, TORRES-VILLALOBOS, Gonzalo, MIRANDA-GALVÁN, Vladimir, ALANIS-MENDIZABAL, Jorge, Manuel ESCOBAR-VALDERRAMA, Juan, NAVA, Cuauhtemoc, Emmanuel DÍAZ-MARTÍNEZ, Néstor, Tatiana BLISKUNOVA, Tatiana, MORALES-DE LOS SANTOS, Victoria Carolina |
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| Zdroj: |
Physiological Research; Oct2024, Vol. 73 Issue 5, p809-817, 9p |
| Abstrakt: |
Primary graft failure occurs 15 to 30 % of the time after transplantation. Although there have been improvements in preserving the lungs in good condition, there have not been studies on the regulation of transcription factors. Methods: We carried out an experimental study involving lung transplantation to indirectly evaluate reactive oxygen species (ROS) production and VEGF expression by competitive blockade of HIF-1a with chetomin. There were 5 groups: Group-1: Lung blocks were perfused with 0.9 % SSF, immediately harvested, and preserved. Group-2 (I-T): Immediate transplantation and then reperfusion for 1 h. Group-3 (I-R): Lung blocks were harvested and preserved in LPD solution for 6 h and reperfused for 1 h. Group-4 (DMSO): Lung blocks were treated for 4 h with DMSO, preserved for 6 h and transplanted to a receptor treated with DMSO. Group-5 (chetomin): Lung blocks were treated for 4 h with chetomin, preserved for 6 h and transplanted to a receptor treated with chetomin. ROS, mRNA, and protein levels of HIF-1a and EG-VEGF were determined. Results: The DMSO and chetomin groups had significantly lower ROS levels. Compared with those in the I-R group, the chetomin group exhibited the lowest level of HIF-1a. Conclusions: Addition of chetomin to the donor and the receptor results in a significant reduction in HIF-1A, VEGF and ROS. [ABSTRACT FROM AUTHOR] |
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