| Autor: |
Yang, Zi-Wen, Liu, Wen-Li, Zhang, Peng, Guo, Dan-Yuan, Wang, Hong-Chuan, Li, Jia-Ling, Wang, Pan-Deng, Dong, Gui-Xin, Nie, Guo-Xing, Li, Wen-Jun |
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| Zdroj: |
Systematic & Applied Microbiology; May2024, Vol. 47 Issue 2/3, pN.PAG-N.PAG, 1p |
| Abstrakt: |
Two novel actinobacteria, designated as SYSU M7M538T and SYSU M7M531, were isolated from oral of Eumetopias jubatus in Zhuhai Chimelong Ocean Kingdom, China. The cells of these microorganisms stained Gram-positive and were rod shaped. These strains were facultative anaerobic, and catalase-positive. Optimal growth occurred at 37 °C and pH 7.0 over 7 days of cultivation. Both strains possessed diphosphatidylglycerol, phosphatidylglycerol and phosphocholine as the major polar lipids. The main menaquinone was MK-9(H 4). The major fatty acids were C 16:0 , C 17:1 w 8 c , C 17:0 , C 18:1 w 9 c and C 18:0. Analyses of genome sequences revealed that the genome size of SYSU M7M538T was 2.1 Mbp with G + C content of 52.5 %, while the genome size of SYSU M7M531 was 2.3 Mbp with G + C content of 52.7 %. The ANI and 16S rRNA gene analysis results showed that the pairwise similarities between the two strains and other recognized Nitriliruptoria species were less than 64.9 % and 89.0 %, respectively. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strains SYSU M7M538T and SYSU M7M531 formed a well-separated phylogenetic branch distinct from other orders of Nitriliruptoria. Based on the data presented here, these two strains are considered to represent a novel species of a novel genus, for which the name Stomatohabitans albus gen. nov., sp. nov., with the type strain SYSU M7M538T (=KCTC 59113T = GDMCC 1.4286T), are proposed. We also propose that these organisms represent a novel family named Stomatohabitantaceae fam. nov. of a novel order Stomatohabitantales ord. nov. [ABSTRACT FROM AUTHOR] |
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