| Autor: |
Laihad, Fanny Margaretha, Titut Harnanik, Juliandhy, Tedy |
| Zdroj: |
International Medical Journal; Apr2021, Vol. 28 Issue 2, p271-273, 3p |
| Abstrakt: |
Objectives: Aspergillosis is one of the fastest growing and deadly fungal infections in humans. Rhinomaxillary or rhinocerebral types are oral manifestations that often occur first after tooth extraction. Hyperbaric oxygen (HBO) is one of the potential therapies for this infection. The aim of this study was to examine the growth of Aspergillus niger strain ATCC®16404TM after fourteen treatments with hyperbaric oxygen in macroscopic and microscopic aspects. Materials and Methods: Four plates of sabouraud agar were cultured with 20 µl 106 CFU / mL of Aspergillus niger strain ATCC®16404TM and incubated for 72 hours at 37°C. The plates were then divided into 2 groups as control and treatment plates, each of which consisted of 2 plates. The control group remained untreated and the treatment group treated with HBO O2 100% 14 times (twice daily for 7 days) 2.4 ATA 3 x 30 minutes. Results: Growth colonies after 72 hours of incubation appeared as black filament colonies on the surface of the Sabouraud agar. After treatment with HBO, the filament colony changed color brighter compared to the control group. Microscopic examination showed fungal morphological damage in the treatment group. This research was analyzed descriptively in macroscopic and microscopic aspects. Conclusion: Hyperbaric oxygen affects the characteristics and inhibits the growth of the Aspergillus niger strain ATCC®16404TM [ABSTRACT FROM AUTHOR] |
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