| Abstrakt: |
In the present work we demonstrated an environmentally acceptable biogenic synthesis of ZnO NPs using Pseudomonas putida (MCC 2989) broth culture. The physiochemical and morphological characters of the synthesized ZnO NPs was confirmed using UV–vis spectroscopy, XRD, SEM-EDX, and FTIR. The anti biofilm and anti bacterial activity of the synthesized ZnO NPs were studied against Pseudomonas otitidis (MCC 2509), Pseudomonas oleovorans (MCC 2566), Acinetobacter baumannii (MCC 2366), Bacillus cereus (MCC, 2039), and Enterococcus faecalis (MCC, 2041) using MTP and disk diffusion assay. The UV–Vis spectra show maximum absorbance of 300 and 310 nm for supernatant and isolated ZnO pellet, respectively, with a corresponding band gap of 4.00 eV. The FTIR results show the evidence for the presence of C O, CH, and NH 2 groups, which proves the presence of remains of carbohydrates and amino acids from the bacterial culture used that is responsible for the formation of nanoparticle as well as for its stabilization. The results of anti-biofilm activity by MEP assay proved that the green synthesized ZnO NPs showed very potent activity against the tested microbial biofilms. Further, the ZnO NPs possess great anti microbial activity against the tested gram (+) and gram (−) ve microbes. Image 1 • ZnO nano particle was synthesized using Pseudomonas putida culture. • Physiochemical and morphological characters of synthesized ZnO Nps were tested using SEM, EDX, XRD and FTIR. • Results of SEM, TEM and XRD analysis evidenced that the particle were agglomerated spherical particle with 44.5 nm in size. • The synthesized ZnO Nps showed potent inhibition of tested microbial cultures and biofilm. [ABSTRACT FROM AUTHOR] |
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