| Autor: |
COZMA, Andreea Paula, MĂCIUCĂ, Iulia Elena, CARP-CĂRARE, Cătălin, RIMBU, Cristina, GUGUIANU, Eleonora, CARP-CĂRARE, Mihai, TIMOFTE, Dorina |
| Předmět: |
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| Zdroj: |
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Veterinary Medicine; 2018, Vol. 75 Issue 1, p133-136, 4p |
| Abstrakt: |
Enterobacteriaceae producing extended-spectrum beta-lactamase (ESBL) enzymes are resistant to betalactam agents and are also commonly multidrug resistant being associated with the resistance to other classes of antibiotics. The aim of our study was to characterise resistance patterns in non-beta-lactam antibiotics of ESBL-producing Enterobacteriaceae strains isolated from faecal matter of pets and owners. The study was carried out on 63 samples of faecal matter (42 from pets and 21 from owners). The ESBL screening was carried out using the Brilliance ESBL Oxoid chromogenic medium. The isolated strains that generated characteristic presumptive ESBL-producing colonies were cultivated on 5% sheep blood medium for the extraction of bacterial DNA using the boiled preps technique. The confirmation of E. coli species was performed molecularly based on the detection of blauidA and blauspA genes. Other Enterobacteriaceae species were identified based on the minimum biochemical characteristics using the MIU and TSI medium. The phenotypical confirmation of presumptive ESBL-producing strains was carried out using the Double Disc Synergy Test (DDST) using a combination of 3rd generation cephalosporins and beta-lactamase inhibitor agents. The determination of the resistance degree in other classes of antibiotics was carried out through the Kirby-Bauer diffusimetric method, and the results were interpreted according to the CLSI standard. Following the species investigation of isolates, 60/63 (95.28%) belonged to the E. coli species and 3/63 (4.72%) to the K. pneumoniae species. Animal isolates were resistant to sulphonamides (54.76% resistance to SXT), fluoroquinolones (45.23% resistance to ENR) and tetracyclines (54.75% resistance to TE). In addition to strains of animal origin for isolates of human origin, an increased resistance has been noticed to phenicols and aminoglycosides. This study has identified a high prevalence of ESBL-producing Enterobacteriaceae strains and associated with multidrug resistance for pets and their owners. [ABSTRACT FROM AUTHOR] |
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