Immunohistochemical expression of nuclear factor [kappa]B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts.

Autor: de Andrade Santos PP, de Aquino AR, Oliveira Barreto A, de Almeida Freitas R, Galvao HC, de Souza LB
Zdroj: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontology; Oct2011, Vol. 112 Issue 4, p476-483, 8p
Abstrakt: OBJECTIVE: The aim of this study was to compare the immunohistochemical expression of nuclear factor [kappa]B (NF-[kappa]B), matrix metalloproteinase 9 (MMP-9), and CD105 in odontogenic keratocysts (OKCs), dentigerous cysts (DCs), and radicular cysts (RCs). STUDY DESIGN: Twenty cases of OKCs, 20 DCs, and 20 RCs were analyzed. A labeling index (LI), which expresses the percentage of NF-[kappa]B-stained nuclei, was calculated for the analysis of NF-[kappa]B expression. Expression of MMP-9 in the epithelium and in the capsule of each lesion was scored as 0 (<10% stained cells), 1 (10%-50% stained cells), or 2 (>50% stained cells). In addition, MMP-9 immunostaining was analyzed in endothelial cells of vessels with a conspicuous lumen. The angiogenic index was determined based on the number of anti-CD105 antibody-stained microvessels. RESULTS: In the epithelial component, the NF-[kappa]B LI was higher in OKCs than in DCs and RCs (P < .001). Analysis of MMP-9 expression in the epithelial component showed a predominance of score 2 in OKCs (90%), DCs (70%), and RCs (65%; P = .159). Evaluation of the NF-[kappa]B LI according to the expression of MMP-9 in the epithelial lining revealed no significant difference between lesions (P = .282). In the fibrous capsule, the highest percentage of MMP-9-stained cells (score 2) was observed in OKCs (P = .100). Analysis of the expression of MMP-9 in the vessels of odontogenic cysts showed a predominance of score 2 in OKCs (80%) and RCs (50%) and of score 1 in DCs (75%; P = .002). Mean microvessel count was high in RCs (16.9), followed by DCs (12.1) and OKCs (10.0; P = .163). No significant difference in microvessel count according to the expression of MMP-9 was observed between groups (P = .689). CONCLUSIONS: The results suggest that the more aggressive biologic behavior of OKCs is related to the higher expression of MMP-9 and NF-[kappa]B in those lesions. The differences in the biologic behavior of the lesions studied do not seem to be associated with the angiogenic index. [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index
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