| Autor: |
Renders, G. A. P., Mulder, L., Lin, A. S., Langenbach, G. E. J., Koolstra, J. H., Guldberg, R. E., Everts, V. |
| Předmět: |
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| Zdroj: |
Dentomaxillofacial Radiology; 2014, Vol. 43 Issue 2, p1-10, 10p |
| Abstrakt: |
Objectives: The temporomandibular joint (TMJ) is susceptive to the development of osteoarthritis (OA). More detailed knowledge of its development is essential to improve our insight into TMJ-OA. It is imperative to have a standardized reliable three-dimensional (3D) imaging method that allows for detailed assessment of both bone and cartilage in healthy and diseased joints. We aimed to determine the applicability of a contrast-enhanced microCT (μCT) technique for ex vivo research of mouse and human TMJs. Methods: Equilibrium partitioning of an ionic contrast agent via μCT (EPIC-μCT) was previously applied for cartilage assessment in the knee joint. The method was ex vivo, applied to the mouse TMJ and adapted for the human TMJ. Results: EPIC-μCT (30-min immersion time) was applied to mouse mandibular condyles, and 3D imaging revealed an average cartilage thickness of 110 ± 16 μm. These measurements via EPIC-μCT were similar to the histomorphometric measures (113 ± 19 μm). For human healthy OA-affected TMJ samples, the protocol was adjusted to an immersion time of 1 h. 3D imaging revealed a significant thicker cartilage layer in joints with early signs of OA compared with healthy joints (414.2 ± 122.6 and 239.7 ± 50.5 μm, respectively). A subsequent significant thinner layer was found in human joints with late signs of OA (197.4 ± 159.7 μm). Conclusions: The EPIC-μCT technique is effective for the ex vivo assessment of 3D cartilage morphology in the mouse as well as human TMJ and allows bone-cartilage interaction research in TMJ-OA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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