| Autor: |
Hegarty, B.C., Bradley, J.M., Lappin, M.R., Balakrishnan, N., Mascarelli, P.E., Breitschwerdt, E.B. |
| Předmět: |
|
| Zdroj: |
Journal of Veterinary Internal Medicine; Jan2014, Vol. 28 Issue 1, p38-41, 4p |
| Abstrakt: |
Background Little is known about the specificity of Bartonella spp. immunofluorescent antibody ( IFA) assays in dogs. Bacteremia in sick dogs most often has been associated with Bartonella henselae ( Bh) , Bartonella vinsonii subspecies berkhoffii ( Bvb), and Bartonella koehlerae ( Bk). Clarification of the diagnostic utility of IFA serology when testing against these organisms is needed. Objective To evaluate the specificity of Bartonella IFA assays utilizing 6 cell culture-grown antigen preparations. Animals Archived sera from SPF dogs (n = 29) and from dogs experimentally infected with Bvb (n = 10) and Bh (n = 3). Methods Antibodies (Abs) to Bvb genotypes I, II, and III, Bh serotype I, strains H-1 and SA2, and to Bk were determined by IFA testing. Results Serum from naïve SPF dogs shown to be negative for Bartonella bacteremia did not react with any of the 6 Bartonella antigens by IFA testing. Dogs experimentally infected with Bvb genotype I developed Abs against homologous antigens, with no cross-reactivity to heterologous Bvb genotypes, Bh H-1, SA2 strains, or to Bk. Dogs experimentally infected with Bh serotype I developed Abs against Bh H-1, but not to Bh SA2 strain with no cross-reactive Abs to Bvb genotypes I- III or to Bk. Conclusions and Clinical Importance Bartonella spp. Ab responses during acute experimental infections are species and type specific. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text