| Autor: |
Neumann, Detlef, Strasser, Andreas, Wesche, Holger, Resch, Klaus, Martin, Michael Uwe |
| Předmět: |
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| Zdroj: |
Cell Death & Differentiation; Nov97, Vol. 4 Issue 7, p580, 10p |
| Abstrakt: |
The CD5[sup +] mouse pre-B cell line SPGM-1 is able to undergo lineage conversion towards a macrophage like cell type. Although one of the κ -Iight chain alleles is rearranged in V-Jκ4 configuration, no protein is expressed nor could the expression be induced. Infection with and expression of the human bcl-2 gene protected SPGM-1 cells from apoptosis, allowing the rearrangement of their second κ-allele in V-Jκ5 configuration, mRNA transcripts of the V-Jκ regions were detected only in SPGM-1 × bcl-2 cells, but not in SPGM-1, while κ-Iight chain protein was not found in any of the cell lines. The myeloid differentiation potential of SPGM-1 cells was not affected by overexpression of the human bcl-2 gene. Upon appropriate stimulation, SPGM-1xbcl-2 cells became enlarged, adhered to the plastic surfaces and lost their immunoglobulin μ heavy chain expression. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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