| Autor: |
Kieleczawa, Jan, Mazaika, Erica |
| Předmět: |
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| Zdroj: |
Journal of Biomolecular Techniques; Jul2010, Vol. 21 Issue 2, p97-102, 6p |
| Abstrakt: |
In this paper, we have fine-tuned a DNA sequencing protocol suitable for a wide range of difficult templates. The primary goal was to evaluate a number of parameters--such as various dye terminator mixes in the presence or absence of additives, the amount of DNAor primer, and cycling protocols--about the effectiveness of reading through complex regions. We showed that the modification of a published protocol leads to significant (75%) cost reduction without forfeiting quality of the data. In the recommended protocol, we used betaine as a standard additive, but better results can be obtained when betaine and Reagent A are mixed in an equivalent ratio. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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