| Autor: |
Bryan, Christopher F, McDonald, Scott B, Baier, Karen A, Luger, Alan M, Aeder, Mark I, Murillo, Daniel, Muruve, Nicolas A, Nelson, Paul W, Shield, Charles F, Warady, Bradley A |
| Předmět: |
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| Zdroj: |
Clinical Transplantation; Oct2002 Supplement 7, Vol. 16, p15, 9p |
| Abstrakt: |
HLA Class I antibody screening can be performed by flow cytometry using a mixture of 30 distinct bead populations each coated with the Class I antigen phenotype derived from different cell lines. In this study we compared the efficacy of Class I antibody screens done by flow cytometry beads with the antihuman globulin (AHG) method for patients awaiting cadaveric renal retransplantation. Class I panel reactive antibody (PRA) screening by flow cytometric beads of 21 regraft serum samples that had all been found to be negative by AHG DTT Class I PRA, revealed that 57.1% (12 of 21) had a flow Class I PRA of ≥ 10%. Furthermore, when five regraft sera with an intermediate PRA were screened (mean AHG DTT PRA = 33.2 ± 13%) the mean flow Class I PRA almost doubled (mean flow PRA = 72.4 ± 10.2%) ( p < 0.01). When active UNOS waiting list regraft candidates, after several months of screening the Class I PRA by flow beads, were divided into the three PRA categories based on their peak flow Class I PRA value (0-20%, 21-79% and ≥ 80%), the incidence of a positive flow cross-match was 0%, 72% and 85% and the incidence of retransplantation was 60%, 22% and 10%, in each of these groups, respectively. These data provided our histocompatibility laboratory with the rationale to stop performing the AHG PRA and perform only the flow Class I PRA method for regraft candidates. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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