| Autor: |
Schwegler, Johann, Heuner, Almut, Silbernagl, Stefan |
| Zdroj: |
Pflügers Archiv: European Journal of Physiology; 1989, Vol. 414 Issue 5, p543-550, 8p |
| Abstrakt: |
A study has been made of electrogenic cellular uptake of amino acids resulting in the depolarization of cell membrane potential (PD) in confluent monolayers of an established opossum kidney (OK) cell line using conventional and pH-selective microelectrodes. Apical superfusion of neutral and dibasic amino acids rapidly depolarized the cell membrane, while application of acidic amino acids had no effect on PD. The depolarization in response to L-phenylalanine and L-arginine was stereoselective, dose-dependent and saturable. 10 mmol/l of L-phenylalanine reduced PD by 4.8±0.4 mV ( n=51) in a completely sodium-dependent way and the concentration necessary for halfmaximal depolarization (C) was about 1.5 mmol/l. On the other hand, the C for L-arginine was about 0.02 mmol/l. The maximal depolarization produced by L-arginine (measured at 10 mmol/l) amounted to 6.8±1.2 mV ( n=10) and this was not affected when extracellular sodium was replaced by choline (6.3±1.2 mV; n=10). The depolarizations induced by L-phenylalanine and L-arginine were significantly additive ( p<0.001). The intracellular pH of OK cells was 7.09±0.03 ( n=11) and did not change during L-arginine application. We conclude that (1) carrier-mediated uptake of neutral and dibasic amino acids into OK cells is at least partially electrogenic. (2) L-Phenylalanine is transported by a Na-symport. (3) In contrast, L-arginine depolarizes PD independently of extracellular sodium. (4) Electrogenic uptake of acidic amino acids is not detectable in OK cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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